Picture Blog: More Efficient Reprogramming for Creating Induced Stem Cells (iPSCs)

Researchers at Allele Biotech achieved reprogramming of human fibroblasts into iPSCs within one week by including mRNA transfection supplement in daily medium change, at “bulk conversion” efficiency, and with cells seeded at a much wider density range compared to our previous publications. These significant improvements will further facilitate high throughput, large scale iPSC production using Allele’s feeder-free, xeno-free, footprint-free reprogramming, which was already a preferred method for both clinical applications and stem cell banking. The reprogramming project is currently being funded by the NIDA/NIH.

Dr. Jiwu Wang’s team at Allele Biotech (and his sons) succeeded on turning human skin cells into stem cells in 7 days. They also used a different medium than previously reported, even without B18R. Although the cells in the 15k cell well died, they are still happy of what they accomplished in all other cell densities tested.

Human skin fibroblasts were reprogrammed into stem cells using a proprietary mRNA cocktail in just a week.

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Thursday, August 1st, 2013 iPSCs and other stem cells

1 Comment to Picture Blog: More Efficient Reprogramming for Creating Induced Stem Cells (iPSCs)

blogadmin
August 4, 2013

It’s easy. The only thing you do is to add a preformed mRNA/transfection reagent mixture to the daily medium change for about a week together with typical stem cell medium supplements such as bFPF.

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