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	<title>AlleleBlog</title>
	<atom:link href="http://blog.allelebiotech.com/feed/" rel="self" type="application/rss+xml" />
	<link>http://blog.allelebiotech.com</link>
	<description>The stories by Allele people and opinions of everyone</description>
	<lastBuildDate>Thu, 03 May 2012 16:27:22 +0000</lastBuildDate>
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		<item>
		<title>Picture Blog — Making mRNAs by In Vitro Transcription for Transgene Expression and R-iPSCs</title>
		<link>http://blog.allelebiotech.com/2012/05/picture-blog-%e2%80%94-making-mrnas-by-in-vitro-transcription-for-transgene-expression-and-r-ipscs/</link>
		<comments>http://blog.allelebiotech.com/2012/05/picture-blog-%e2%80%94-making-mrnas-by-in-vitro-transcription-for-transgene-expression-and-r-ipscs/#comments</comments>
		<pubDate>Thu, 03 May 2012 05:01:14 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Uncategorized]]></category>
		<category><![CDATA[iPS and other stem cells]]></category>
		<category><![CDATA[feeder-free]]></category>
		<category><![CDATA[footprint free]]></category>
		<category><![CDATA[huma iPS]]></category>
		<category><![CDATA[IVT]]></category>
		<category><![CDATA[mRNA]]></category>
		<category><![CDATA[R-iPSCs]]></category>
		<category><![CDATA[transgene]]></category>
		<category><![CDATA[Warren]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1442</guid>
		<description><![CDATA[Transgene expression by introducing in vitro transcribed mRNA can give you footprint free, high level, an uniform expression in even hard-to-transfect cells like primary fibroblasts.  The trick is to know how to modify the mRNAs and what dose to use.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2012/05/picture-blog-%e2%80%94-making-mrnas-by-in-vitro-transcription-for-transgene-expression-and-r-ipscs/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Picture Blog &#8212; Human mRNA-Induced Pluripotent Stem Cells Generated in Days</title>
		<link>http://blog.allelebiotech.com/2012/04/picture-blog-human-mrna-induced-pluripotent-stem-cells-generated-in-days/</link>
		<comments>http://blog.allelebiotech.com/2012/04/picture-blog-human-mrna-induced-pluripotent-stem-cells-generated-in-days/#comments</comments>
		<pubDate>Sun, 22 Apr 2012 03:42:46 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[iPS and other stem cells]]></category>
		<category><![CDATA[Episomal plasmids]]></category>
		<category><![CDATA[footprint free]]></category>
		<category><![CDATA[human ESCs]]></category>
		<category><![CDATA[human iPSCs]]></category>
		<category><![CDATA[Luigi Warren]]></category>
		<category><![CDATA[mRNA transfection]]></category>
		<category><![CDATA[R-iPSC]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1421</guid>
		<description><![CDATA[Footprint-free human iPSCs can be produced in as short as only a few days--if you know how to do it right.  The process can potentially also be xeno-free for clinical grade stem cells.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2012/04/picture-blog-human-mrna-induced-pluripotent-stem-cells-generated-in-days/feed/</wfw:commentRss>
		<slash:comments>2</slash:comments>
		</item>
		<item>
		<title>Using Insect Cells For Making Mammalian Proteins</title>
		<link>http://blog.allelebiotech.com/2012/02/using-insect-cells-for-making-mammalian-proteins/</link>
		<comments>http://blog.allelebiotech.com/2012/02/using-insect-cells-for-making-mammalian-proteins/#comments</comments>
		<pubDate>Wed, 29 Feb 2012 22:26:45 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Viruses and cells]]></category>
		<category><![CDATA[baculovirus]]></category>
		<category><![CDATA[BVES]]></category>
		<category><![CDATA[insect cells]]></category>
		<category><![CDATA[insoluble proteins]]></category>
		<category><![CDATA[mammalian proteins]]></category>
		<category><![CDATA[protein expression]]></category>
		<category><![CDATA[PTM]]></category>
		<category><![CDATA[sf21]]></category>
		<category><![CDATA[sf9]]></category>
		<category><![CDATA[T. ni]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1364</guid>
		<description><![CDATA[For protein expression in insect cells, a number of factors need to be taken into consideration: 1) Genomic DNA for creating baculovirus stocks that will ensure...]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2012/02/using-insect-cells-for-making-mammalian-proteins/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Opportunities for business with Allele Biotech</title>
		<link>http://blog.allelebiotech.com/2012/02/opportunities-for-business-with-allele-biotech/</link>
		<comments>http://blog.allelebiotech.com/2012/02/opportunities-for-business-with-allele-biotech/#comments</comments>
		<pubDate>Wed, 08 Feb 2012 23:31:05 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Allele Mail Bag]]></category>
		<category><![CDATA[Customer Feedback]]></category>
		<category><![CDATA[Open Forum]]></category>
		<category><![CDATA[beauty products]]></category>
		<category><![CDATA[chicken feed]]></category>
		<category><![CDATA[cosmetic]]></category>
		<category><![CDATA[diagnosis]]></category>
		<category><![CDATA[farm animals]]></category>
		<category><![CDATA[skin care]]></category>
		<category><![CDATA[swine flu]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1359</guid>
		<description><![CDATA[Allele Biotech is known for staying on the edge of biological research fronts when it comes to developing new technologies into useful tools. Our research also has far-reaching implications and potential applications outside of the traditional biomedical research reagent field.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2012/02/opportunities-for-business-with-allele-biotech/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Making Transfection-Grade mRNA by IVT (In Vitro Transcription)</title>
		<link>http://blog.allelebiotech.com/2012/01/making-transfection-grade-mrna-by-ivt-in-vitro-transcription/</link>
		<comments>http://blog.allelebiotech.com/2012/01/making-transfection-grade-mrna-by-ivt-in-vitro-transcription/#comments</comments>
		<pubDate>Thu, 12 Jan 2012 00:37:42 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Fluorescent proteins]]></category>
		<category><![CDATA[Viruses and cells]]></category>
		<category><![CDATA[iPS and other stem cells]]></category>
		<category><![CDATA[fluorescent protein]]></category>
		<category><![CDATA[iPS]]></category>
		<category><![CDATA[IVT]]></category>
		<category><![CDATA[luciferase]]></category>
		<category><![CDATA[mRNA]]></category>
		<category><![CDATA[nuclease]]></category>
		<category><![CDATA[recombinase]]></category>
		<category><![CDATA[RiPSCs]]></category>
		<category><![CDATA[RNA degradation]]></category>
		<category><![CDATA[RNase]]></category>
		<category><![CDATA[TALEN]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1351</guid>
		<description><![CDATA[RNases are an often feared in molecular biology labs because of their high stability and ominous presence in virtually all living systems. But that's no reason to be afraid of using IVT mRNA as the most efficient way of mediating expression of iPS, nuclease, recombinase, luciferase, fluorescent protein, etc.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2012/01/making-transfection-grade-mrna-by-ivt-in-vitro-transcription/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Record number of papers citing the GFP-Trap group products in 2011</title>
		<link>http://blog.allelebiotech.com/2012/01/record-number-of-papers-citing-gfp-trap-line-of-products-in-the-second-half-of-2011/</link>
		<comments>http://blog.allelebiotech.com/2012/01/record-number-of-papers-citing-gfp-trap-line-of-products-in-the-second-half-of-2011/#comments</comments>
		<pubDate>Thu, 05 Jan 2012 00:14:49 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Camelid Antibody]]></category>
		<category><![CDATA[Uncategorized]]></category>
		<category><![CDATA[anti-GFP]]></category>
		<category><![CDATA[camelid antibodies]]></category>
		<category><![CDATA[Chromotek]]></category>
		<category><![CDATA[Co-IP]]></category>
		<category><![CDATA[GFP-Trap]]></category>
		<category><![CDATA[GFPTrap]]></category>
		<category><![CDATA[immunoprecipitation]]></category>
		<category><![CDATA[pulldown]]></category>
		<category><![CDATA[RFPTrap]]></category>
		<category><![CDATA[VHH]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1334</guid>
		<description><![CDATA[In 2011 GFP-Trap continued to be adopted by an increasing number of labs as the reagent of choice for immunoprecipitation.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2012/01/record-number-of-papers-citing-gfp-trap-line-of-products-in-the-second-half-of-2011/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Year-end message from Allele Biotech</title>
		<link>http://blog.allelebiotech.com/2011/12/year-end-message-from-allele-biotech/</link>
		<comments>http://blog.allelebiotech.com/2011/12/year-end-message-from-allele-biotech/#comments</comments>
		<pubDate>Sat, 31 Dec 2011 21:19:55 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Uncategorized]]></category>
		<category><![CDATA[2012]]></category>
		<category><![CDATA[EGFP]]></category>
		<category><![CDATA[iPS]]></category>
		<category><![CDATA[iPSC]]></category>
		<category><![CDATA[mRNA templates]]></category>
		<category><![CDATA[NCI SBIR]]></category>
		<category><![CDATA[new fluorescent proteins]]></category>
		<category><![CDATA[reposting]]></category>
		<category><![CDATA[retweeting]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1340</guid>
		<description><![CDATA[The year 2011 has been an exciting and eventful year for many people.  Throughout the year, we have been working diligently to bring the best research methods in many areas to our fellow researchers through innovation and entrepreneurship.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2011/12/year-end-message-from-allele-biotech/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Top 10 List of Most Viewed AlleleBlogs in 2011</title>
		<link>http://blog.allelebiotech.com/2011/12/top-10-list-of-most-viewed-alleleblogs-in-2011/</link>
		<comments>http://blog.allelebiotech.com/2011/12/top-10-list-of-most-viewed-alleleblogs-in-2011/#comments</comments>
		<pubDate>Sat, 31 Dec 2011 00:43:54 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[Allele Mail Bag]]></category>
		<category><![CDATA[camelid antibodies]]></category>
		<category><![CDATA[environment friendly research]]></category>
		<category><![CDATA[GFP-Trap]]></category>
		<category><![CDATA[iPS]]></category>
		<category><![CDATA[mRNA iPS]]></category>
		<category><![CDATA[RiPSC]]></category>
		<category><![CDATA[RNAi screening]]></category>
		<category><![CDATA[shRNA lentivirus packaging]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1335</guid>
		<description><![CDATA[The ballot is in—among the “usual suspect” hot topics, iPS takes the top honor and most entries;  Camelid antibodies, although not really presented as a typical AlleleBlog in 2011, made it to the top 3.  shRNA cloning and RNAi screening are still on a lot of people’s minds, so it seems.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2011/12/top-10-list-of-most-viewed-alleleblogs-in-2011/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>How do you make shRNA-expressing viruses for function screening?</title>
		<link>http://blog.allelebiotech.com/2011/11/how-do-you-make-shrna-expressing-viruses-for-function-screening/</link>
		<comments>http://blog.allelebiotech.com/2011/11/how-do-you-make-shrna-expressing-viruses-for-function-screening/#comments</comments>
		<pubDate>Thu, 17 Nov 2011 00:46:44 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[RNAi patent landscape]]></category>
		<category><![CDATA[lentivirus packaging]]></category>
		<category><![CDATA[lentiviurs shRNA]]></category>
		<category><![CDATA[RNAi]]></category>
		<category><![CDATA[RNAi screening]]></category>
		<category><![CDATA[shRNA]]></category>
		<category><![CDATA[shRNA cloning]]></category>
		<category><![CDATA[shRNA lentivirus packaging]]></category>
		<category><![CDATA[shRNA library]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1331</guid>
		<description><![CDATA[Most people use standard cloning procedures when trying to insert shRNA templates into lentiviral vectors.  However, since typical  lentiviral vector plasmids have terminal repeats and are relatively large, when ligated to hairpin sequence-containing shRNA templates, recombination often occurs inside bacteria that results in smaller plasmids.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2011/11/how-do-you-make-shrna-expressing-viruses-for-function-screening/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
		</item>
		<item>
		<title>Development of Cell Lines from iPSCs for Bioassays</title>
		<link>http://blog.allelebiotech.com/2011/11/development-of-cell-lines-from-ipscs-for-bioassays/</link>
		<comments>http://blog.allelebiotech.com/2011/11/development-of-cell-lines-from-ipscs-for-bioassays/#comments</comments>
		<pubDate>Wed, 09 Nov 2011 20:03:11 +0000</pubDate>
		<dc:creator>blogadmin</dc:creator>
				<category><![CDATA[iPS and other stem cells]]></category>
		<category><![CDATA[cardiomyocyte]]></category>
		<category><![CDATA[didifferentiation]]></category>
		<category><![CDATA[direct reprogramming]]></category>
		<category><![CDATA[Gata4]]></category>
		<category><![CDATA[iCM]]></category>
		<category><![CDATA[iN]]></category>
		<category><![CDATA[iPSC]]></category>
		<category><![CDATA[Mef2c]]></category>
		<category><![CDATA[redifferentiation]]></category>
		<category><![CDATA[Tbx5]]></category>
		<category><![CDATA[transdifferentiation]]></category>

		<guid isPermaLink="false">http://blog.allelebiotech.com/?p=1328</guid>
		<description><![CDATA[Technologies are being developed to produce re-differentiated cells of a number of lineages.  Transdifferentiation, or direct reprogramming, by introducing a group of 3 cardiomyocyte-specific factors, investigators could directly program cardiac or dermal fibroblasts into cardiomyocyte-like cells.]]></description>
		<wfw:commentRss>http://blog.allelebiotech.com/2011/11/development-of-cell-lines-from-ipscs-for-bioassays/feed/</wfw:commentRss>
		<slash:comments>0</slash:comments>
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