Allele Biotech Spotlight Promo for ASCB Dec 09 Meeting!

This year our President and CEO, Dr. Jiwu Wang Ph.D., will be presenting at the American Society for Cell Biology meeting in San Diego, December 5th through 9th. Dr. Wang will be presenting results of two studies that involved the Allele Biotech Fluorescent Proteins and iPSC product lines:

Monomeric photoconvertable fluorescent protein variants produced by directed evolution for brightness and efficient photoconversion – a collaborative effort with the Campbell lab at the University of Alberta

Increased efficiency and speed of reprogramming of human cells into induced stem cells using high-titer lentiviral vectors encoding cell cycle progression and survival genes – a collaborative effort with the Chang lab at the University of Florida

In honor of this prestigious occasion Allele Biotech is having a Spotlight Promotion on all Fluorescent Protein and iPSC Products! The promotions, which will vary from product to product, will include 10% and 20% off price reductions, FREE shipping, and even “Buy 2 get one Free” deals!

Products eligible for the Spotlight Promotions begin with:

ABP-FP-____ Catalog

ABP-SC-____ Catalog

To qualify for these promotions you must be attending the ASCB meeting in San Diego and provide us with a copy of your registration form or be one of our loyal facebook, twitter, or myspace friends. Any questions can go to oligo@allelebiotech.com

Call for details and ask for info on the Spotlight Promotions! Offers good now through December, 9th 2009!

New Product of the Month 11/23-29/09: ThermoExp500 PCR machine (thermocycler) $4,250.00, with almost twice as fast temperature ramping as MJ’s TC1000, and more reliability.

Tags: Allele Biotech Promotions, Allele Biotech Sales, American Society for Cell Biology, ASCB San Diego 2009, fluorescent proteins discount, free shipping, iPSC discount, MJ research, PCR machine, TC1000, thermocycler

Competition from the Marketplace to the Courtroom

The hottest subject in the biological research equipment field has to be whole genome sequencing; hence it is no surprise that companies execute mergers and acquisitions in order to position themselves to go after their competitors in an attempt to corner this valuable market.

A bit of the background history: Illumina was started a decade ago to build DNA chip arrays by people with experience at Affymetrix, when the latter was the first and absolute leader in the DNA chip field. For years, rather than providing DNA chips, Illumina was known for generating revenue by selling oligonucleotides at 20% of the prevailing market price, essentially starting the low end oligo market. Just three or four years ago, it was a front page promotion on Invitrogen’s website to sell Illumina’s oligos through a production/shipping alliance, a cooperation previously unheard of in our field for such low price, non-commodity products. This move quite probably contributed to the decisions made by the more dedicated oligo company, IDT, to acquire local oligo production houses and move to the West coast (Allele opted out of such an acquisition and later did one of its own by taking over Orbigen and since moved into the viral systems and antibody fields). At that point when whole genome sequencing technologies were becoming mature and marketable, Illumina had performed brilliantly in out competing the previously dominant chip supplier Affymetrix, acquired Solexa, and quickly moved into the whole genome sequencing with Genome Analyzer and Genome Analyzer II, a move Affi’s management probably regretted not making.

In the years roughly around 2005-2007, Applied Biosystems, Inc. (ABI) was developing its own genome analysis equipment, the SOLiD system. It surely had a solid base to build on from its strong leadership in providing sequencer and analyzers for many years. Earlier in the year Invitrogen and ABI merged to form Life Technologies, pitching Invitrogen (now LifeTech) and Illumina in a collision course in battle for dominance in genomic analysis. In September, LifeTech brought suit against Illumina for patent infringement; in October Illumina countered with suits of its own. While the fight in court may be long and only sprinkled with occasional fireworks, the competition in the market could be fierce and should ultimately decide on whose technology is superior and offered at better prices. From the technical presentation made by sales teams to us during on site seminars, Solexa’s science sounded better. I was sitting next to Jay Flatley, CEO of Illumina at a San Diego biotech CEO dinner, and heard him predicting that the technology would advance and in a few years, one could get their own genome sequenced for about a thousand dollars, ~10% of the current cost! That’s simply innovation and competition at work. But watch out, a new wave of sequencing technologies based on single molecule capture might make the Illumina and LifeTech courtroom argument a moot point in the market.

Tags: biotech, Genome Analyzer, Illumina, Life Technologies, oligo, SOLiD, whole genome sequencing

The economy recession is most likely over, says who?

The economy recession is most likely over, or so says the federal reserve chairman Ben Bernanke. Do you feel it? Are you seeing increased job opportunities when you leave your current lab or security if you have a post-postdoc position? In our industry, where the health of the economy is mostly measured by research budgets of individual labs or research groups, occasionally by budgets for contracting or licensing fees, the change, if any, is still hard-to-find. But hiring at academic institutes like UCSD seems to have picked up lately, probably due to addition grants from the Obama administration’s stimulus programs. At the same time, individual NIH R1 grants have been creeping up to easily around 1 million a year, program grants 3-5 millions. With more stimulus money kicking in to academic labs this fall, it is expected that the situation will further improve. Comments welcome.

Notes about recent jobs in Pharma/Biotech: since our last blog about massive Pfizer layoff of scientists in 02-09-09, a major layoff in the big pharma sector came from Merck, which announced on 06-11-09 that it would cut 16,000 jobs after completing its merger with Shering Plough. On 09-14-09, Eli Lilly reported job cots of 5,500 or roughly 14% of its work force. There are areas in the country where people report about the economy as “I went to 2 grocery stores and 3 discount department stores over one weekend, and you could do cannon shooting practice in there without hitting a person.” Again comments welcome here, if you believe in a turnaround, or it is all doom and gloom to you. Btw, the History channel has been cranking up the 2012 theories for a couple of months now, if you like the doom and gloom theories.

Note added in proof: As reported in Science yesterday, “a new analysis of the grantsmaking process at the National Institutes of Health (NIH) lifts the veil on how many grant proposals are funded even though they fall below a cutoff based on peer-review scores…at least 19% of NIH’s basic research portfolio is funded for reasons that go beyond quality.”

Feeder Cells for Stem Cells

Allele’s entire iPSC product line is designed for the ease of the researcher. Each component in our iPSC catalog will shave priceless time off your protocol by eliminating the tedious steps in iPS induction so you can get down to work.

Allele is adding a major component to its iPSC line: pre-irradiated, ready-to-use, system specific, bFGF-Producing Feeder Cells for iPSC propagation!

Using Allele’s bFGF-Producing Feeder Cells avoids the usual problems associated with MEF cell lines. They are maintained at low passages, come pre-irradiated and ectopically express bFGF so there is no need to supplement your medium with additional growth factors.

Additionally, Allele Biotech is introducing human fibroblasts to the market for iPSC work. MEF is good for mouse iPSC reprogramming but human fibroblast feeders are preferred when creating human iPSCs due to their secreted factors. Propagate human iPSC with greater efficiency while eliminating non-human cells for therapeutic use of human iPSCs!

As always we encourage customer feed back. We are interested to hear about your stem cell work, needs, and requests for new products. We also welcome those who have new ideas and potential products to collaborate with us. We are here to help advance your research and get your technologies to the public.

If you are enjoying AlleleNews and AlleleBlogs: come back and check out our new Forum and FAQ Sections soon to be added to our blogs for quick product/service related exchange and messages of more user control.

Tags: ectopically expressed bFGF, feeder cells, human fibroblast, human iPSC, iPSC induction, optimized iPSC generation, pre-irradiated MEF cells, research help, system specific feeder cells, time efficient iPS generation

Allele Pre-packaged, Titer-determined, Validated iPS Generating Lentivirus Particles

Induced pluripotent stem cell (iPSC) has been an important research area in the past 3 years. These cells have also provided unprecedented possibilities to study cell differentiation and tissue development to biologists in many fields. Allele pre-packaged iPS lentiviral particles are validated, ready-to-use, high quality reagents for any laboratory to create iPS cells. A straight-forward and optimized protocol is also provided for immediate use of these products. iPC cells can be produced typically in 2-3 weeks; but when used in combination with shRNA against p53 (also available from Allele), iPSCs can be produced in just about 5 days!

Follow iPS news on our news page.

Tags: iPS, lentiviral particles, lentivirus, optimized protocol for inducing iPSC, Pre-packaged viral particles, quick iPS induction, titer-determined, validated iPSC