mouse tail genotyping

New SurfaceBind gDNA Isolation and Purification

Allele Biotech’s SurfaceBind Genomic DNA Pu¬rification Kit is designed for fast, easy, and high-throughput gDNA isolation and purification for lysate obtained through the use of Allele-in-One Mouse Tail Direct Lysis Buffer. Based on our Solid Surface Revers¬ible Binding (SSRB) technology the SurfaceBind system utilizes a plastic tube with its surface coated with proprietary turbo-binders acting to selectively capture and efficiently bind DNA mol¬ecules from reaction mixtures. After lysis of cells, gDNA molecules will specifically interact with the turbo binders and bind to the surface of the tube in the presence of the binding buffer, while pro¬teins and other contaminants will remain in solu¬tion. The DNA can be eluted with as little as 10 microliters of water or buffer for the next application, allowing for a highly concentrated solution.

The entire process of recovery takes less than 10 minutes with only 1 centrifugation step, making it fast and easy. SSRB technology also provides for maxi¬mum DNA capture and release with limited sam¬ple input, without the DNA loss associated with membrane and bead-based technologies.

This is a newly developed product particularly for the Allele Biotech’s customers who use the All-in-One mouse tail genotyping kits: get purified genomic DNA using the same lysate you generated for a quick PCR. The yield and purity will enable direct applications to chip assays, sequencing, Southern blotting, etc.Next time you use Allele-in-One Mouse Tail Direct Lysis Buffer be sure to try our SurfaceBind gDNA Purification kit.

  • New Product of the Week 011711-012311:

    • mWasabi-GFP Expression vector with IRES for co-expression. Cat # ABP-FP-WIRES10. email FP@allelebiotech.com for more FP IRES-containing plasmids.

  • Promotion of the Week 011011-011811:

    • 10% off all mTFP1-expressing plasmids this week, check out the vectror you like at shop.allelebiotech.com

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    Wednesday, January 19th, 2011 Customer Feedback, Open Forum No Comments

    Your Feedback Helps Us Move Forward

    It seems like every week Allele Biotech has a blog entry introducing a new product, or some cutting edge research. What is often overlooked is the driving force toward this innovation, and that force is you, the customer. As a company we work to make products that address our customer needs while maintaining competitive pricing.

    In order to continue these practices we rely on customer feedback. What differentiates Allele Biotech from other companies is how we react to customer input, we use it to innovate. A great example of this is Allele Mouse Tail Lysis Buffer, a product we created at the request of a customer. This customer wanted an alternative to using phenol, a potentially hazardous chemical. We were able to create our buffer which works efficiently and effectively to lyse DNA safely. Today Mouse Tail Lysis Buffer is one of our top selling reagents, but we still love to hear back from our customers about it. Other examples of customer initiated products are our Cre Lentivirus, and our Protein extraction buffer. The Cre Lentivirus was requested to give the end user a high titer virus stock for applying Cre recombinase to their MEFS. Protein Extraction Buffer was created at the behest of a customer who wanted a low cost, but high quality alternative to B-Per.

    So next time you are in lab and realize you need something, or can improve upon a product send us an email. If you are already using something from Allele let us know if the protocol is unclear, or if it’s too long. Tell us if it was an easy product to use, or if it was a tedious process that can be improved upon. We want to know that our products are working for you, and that you are satisfied with your purchase from Allele Biotech. With this back and forth between company and customer we can build the ideal client relationship.

    New Product of the Week 070510-071110: Pre-cutted pLICO-RNAi-Reporter, 20 reactions, ABP-HL-SE30020 $599.00

    Promotion of the Week 070510-071110: buy Sapphire baculo genomic DNA get insect cells at half price.

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    Wednesday, July 14th, 2010 Customer Feedback No Comments