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A Novel pro-adipogenesis factor abundant in adipose tissues and over-expressed in obesity acts upstream of PPARg and C/EBPa
Yuhui Ni, Chenbo Ji, Bin Wang, Jie Qiu, Jiwu Wang, Xirong Guo
An important question about adipogenesis is how master adipogenesis factors (defined as being able to initiate adipogenesis when expressed alone) peroxisome proliferator-activated receptor (PPAR) initiate adipogenesis only in differentiating preadipocytes. The objective of our research was to find previously unidentified factors that are unique or highly enriched in cells of the adipocyte lineage during adipogenesis that may provide functional tissue specificity to preadipocytes. We reasoned that such factors may alter expression profile specifically in obese individuals. Omental adipose tissues were obtained from obese and non-obese male patients undergoing emergency abdominal surgery. mRNAs extracted from either group were used for suppression subtraction hybridization (SSH). Genes corresponding to mRNAs enriched in obese versus non-obese patients were identified through sequencing and further analyzed for tissue distribution. Out of ~20 genes, we found several that showed clear fat cell specific expression patterns. In this study, we functionally studied one of these genes, previously designated as open reading frame C10orf116. Our data demonstrated that C10orf116 is highly expressed in adipose tissue and is localized primarily within the nucleus. Over-expression studies in 3T3-L1 cells indicated that it up-regulates the levels of CCAAT/enhancer binding protein a (C/EBPa) and PPARg and promotes adipogenic differentiation starting from the early stage of adipogenesis. Over-expressed in omental tissues from obese patients, C10orf16 manifested the characteristics of an adipocyte lineage-specific nuclear factor that can modulate the master adipogenesis transcription factors early during differentiation. Further studies of this factor should help reveal tissue-specific events leading to fat cell development at the transcriptional level.
Link to the original publication: http://link.springer.com/article/10.1007%2Fs10863-012-9492-6
Here is Allele Biotech’s webpage showing relevant cell and development biology products: www.allelebiotech.com
We’re happy to announce that after a long construction and moving process, Allele Biotech has passed its final inspection hurdle and is now officially open for business at our new location, a two-story building we recently purchased on Nancy Ridge Drive. This facility is located at the heart of the Sorrento Mesa biotechnology cluster and contains two floors of brand new lab space to support production and R&D, plus plenty of room for expansion. We’re also welcoming two tenants, Nano CELLect and MesaTech, as well as the non-profit research institute we helped launch earlier this year, the Scintillon Institute (www.scintillon.org).
For our local colleagues and valued customers, please feel free to contact us to schedule a personal tour of our new facility and to meet with our esteemed group of experts who are always willing to discuss in person, your scientific needs
Keep an eye out on this blog for important dates, promotions and more news and photos from the new facility as we prepare for our upcoming open house! Hope to see many of you then if not sooner!
This week in the journal Scientific Reports (Nature Publishing Group) scientists from Allele Biotechnology describe an important advance in the generation of stem cells capable of producing all the different tissues of the human body. In an article entitled “Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA,” Allele’s scientists present the fastest and safest method yet for converting ordinary human skin cells into “induced pluripotent stem cells” (iPSCs).
The scientific efforts were led by Dr. Luigi Warren, whose pioneering work on “footprint-free” reprogramming using messenger RNA was the foundation for Allele’s breakthrough. Through the united efforts of Dr. Warren and the scientists at Allele Biotechnology, his technique was re-engineered to increase cell conversion efficiency and eliminate any use of potentially unsafe reagents, while substantially reducing the time and effort needed to make stem cells. Dr. Warren believes that because of its advantages this technology “should become the method of choice for iPSC cell banking.”
According to Dr. Jiwu Wang, corresponding author on the paper and CEO of Allele Biotechnology, “This advance in stem cell derivation will enable both fundamental scientific research and clinical applications which has been the mission of Allele Biotechnology from its inception.”
Allele Biotechnology and Pharmaceuticals Inc. is a San Diego-based biotechnology company that was established in 1999 by Dr. Jiwu Wang and colleagues. A research based company specializing in the fields of RNAi, stem cells, viral expression, camelid antibodies and fluorescent proteins; Allele Biotechnology has always striven to offer products and services at the cutting edge of research.
Allele Biotechnology and Pharmaceuticals Inc.
Jiwu Wang, Ph.D., 858-587-6645 Ext 3
President and CEO
Press release by BusinessWire. Also see Yahoo!News, Reuters, The Herald, etc.
Allele Biotech is known for staying on the edge of biological research fronts when it comes to developing new technologies into useful tools. Our research also has far-reaching implications and potential applications outside of the traditional biomedical research reagent field. Some of these technologies were the results of researchers interacting with the Allele scientific team, who wanted Allele to help realize their potentials. If you are interested in investing, co-developing, or trading in our areas of expertise, please email us at firstname.lastname@example.org.
1) A novel method of discriminating and/or detecting mismatched polynucleotide populations in a sample, or determining the relative abundance of the species contained in the sample based on the changes in the relative ratios following a critical treatment. This technology, subject of a current patent application, can provide great benefits in polynucleotide-based diagnosis.
2) A technology on how to utilize the light-absorbing capabilities of certain light-absorbing proteins against damaging lights, or in cosmetic or beauty products. It is also a subject of a filed full patent.
3) Products that relate to detecting swine flu with novel antibodies of high specificity and stability. The antibodies have been tested in academic molecular biology labs in ELISA and strip formats.
4) Nanotechnology products that can be immediately applied to prevent citrus diseases on farms.
5) Enzymes as additives to animal feeds that help farm animals digest. The product is already being sold in certain regions.
The ballot is in—among the “usual suspect” hot topics, iPS takes the top honor and most entries; Camelid antibodies, although not really presented as a typical AlleleBlog in 2011, made it to the top 3. shRNA cloning and RNAi screening are still on a lot of people’s minds, so it seems.
Method: total visits to each blog since our new webpage was launched in July was counted.
1) Fusion of the Transcription Domain to iPS Factors Radically Enhances Reprogramming
2) Methods of iPSC Generation Update
3) About 50 Papers Cited the Use of GFP-Trap Camelid Antibody So Far in 2011
4) Big Potential in Using Protozoans for Producing Mammalian Proteins
5) How do you make shRNA-expressing viruses for function screening?
6) Creating ground-state human iPSCs
7) Recombinase-Mediated Cassette Exchange (RMCE) and Integrase Swappable in vivo Targeting Element (InSITE)
Development of Cell Lines from iPSCs for Bioassays
9) Choosing siRNA, shRNA, and miRNA for Gene Silencing
10) Allele Biotech’s Box Swap Program
Have a successful 2012!
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