Triple Labeled Oligos-Put Your Fluorescence in Perspective!

Allele is now distributing to its customers the newest technology in RT-PCR published less than two weeks ago. It is a RT PCR probe that utilizes fluorescence resonance energy transfer (FRET) and TaqMan methods in order to provide an internal positive control (IPC) for real-time PCR assays. This new RT-PCR method consists of forward and reverse primers, a regular TaqMan probe, and the new triple-labeled FRET-TaqMan Probe Probe affixed to a plasmid, which negates the uncertainty in the presence and effects of amplification inhibitors that could generate a false negative in your experiments. Two emission sources are all that are required for the FAM and Cy5.5 fluorescence signals at 530 nm and 705 nm respectively. You do not need color compensation software, multiple excitation sources, or even a high tech laser to initiate excitation with this system.

If your RT PCR instrument only has one light source to excite the fluorophore then the FRET-TaqMan method is the only way to exploit the IPC technology. Fortunately Allele Biotech is selling these triple-labeled probes at a great price! Put your fluorescence in perspective and know your true RT-PCR results with these Specialty Oligos from Allele Biotech!

Tags: fluorescence signal, FRET Probe, FRET TaqMan Probe, internal positive control, IPC, led excitation, multiplex real-time PCR, probe, real-time PCR, RT-PCR, special oligonucleotides, TaqMan Probe, tripple-labeled oligos

Launch of Allele’s Newest Product Group: Special Chemicals

It has only 4 products now, will be a dozen soon, then a few hundreds to thousands in a few months if all goes well. If not, just the oligo chemicals available now should already provide tremendous value to oligo producers because iPCR, Real-time PCR are getting more and more used, requiring large number of probes with these modifications we products can do, but at previously unthinkable low prices. Read about it here.

Tags: amino group, biotin, blocking elongation, capturing oligos, Dabcyl, Dabsyl, FAM, hybridization, iPCR, matching sequences, oligos, probes, real-time PCR